@text   Protein Science 1994 - Vol. 3, Issue 10
"SEQUENCE DETERMINANTS OF THE CAPPING BOX, A STABILIZING MOTIF AT THE N-TERMINI OF ALPHA-HELICES"
Jeffrey W. Seale, Rajgopal Srinivasan, and George D. Rose

		Contents of file SEALE.KIN:
Kin.1- Three superimposed capping boxes (with Ser or Thr N-caps)
Kin.2- A capping box with an Asn N-cap
Kin.3- A "near" capping box
Kin.4- Two superimposed "big" capping boxes

	Kinemage 1 shows three superimposed examples of classic capping boxes at the beginning of alpha helices (from myoglobin, Trp repressor, and carbonic anhydrase).  In each case the N-cap residue (which is half in and half out of the helix), either Ser or Thr here, forms a sidechain H-bond to the exposed backbone NH of residue N3 (N-cap + 3), while the sidechain O of residue N3 forms an H-bond to the backbone NH of the N-cap residue.  This ring of reciprocal H-bonds is shown with seagreen sidechains, purple H-bonds, and red or blue balls for the N and O atoms of the H-bonds.  Surrounding the H-bond ring of the capping box is a hydrophobic interaction between the sidechains of residues N' (N-cap - 1) and N4, shown in gold.  In Trp repressor, residue N4 is an Arg, which makes a hydrophobic interaction with the aliphatic part of its sidechain while the guanidium group H-bonds to the N' carbonyl.

	Kinemage 2 shows a helix capping box (from cytochrome C) with an Asn at the N-cap position, which is less common than with Ser or Thr.  The same H-bond and hydrophobic interactions are made, but with a slight difference in conformation at the N-cap.

	Kinemage 3 shows a "near" capping box (from human lysozyme), which misses forming one of the two H-bonds, in this case the one from the N3 Asn.  The separation is best seen in View2, looking down the helix axis.

	Kinemage 4 shows two superimposed examples of "big" helix capping boxes (from myoglobin and dihydrofolate reductase), for which the N3 H-bond goes to the backbone of residue N' instead of N-cap, and the hydrophobic sidechain is at N" instead of N'.

  Coordinates from Brookhaven Data Bank files: 1MBO, 2WRP, 2CA2, 5CYT, 1LZ1, 4DFR

   Copyright (c) 1994 The Protein Society.  This kinemage is published as part of Protein Science Vol. 3, Issue 10.  All rights reserved.  This and other kinemages published in Protein Science may be reproduced without permission by teachers and students for research and educational purposes providing this notice remains a part of the kinemage file.  In case of doubt or for any other use, contact Cambridge University Press.

@kinemage 1 
@caption
Myoglobin Ser3, Trp repressor Thr44, and carbonic anhydrase Ser219 - alpha helices with classic capping boxes at their N-termini.  Hydrophobic sidechains are in gold, polar ones in seagreen; N and O atoms of the cap box H-bonds are highlighted with color-coded balls.
@onewidth 
@viewid {cap box}
@zoom 1.45
@zslab 110
@center 7.905 12.810 17.909
@matrix
.258648 -.940144 -.221876 -.582523 .031414 -.812201 .770564 .339322 -.53954
@2viewid {helix end}
@2zoom 1.45
@2zslab 140
@2center 7.905 12.810 17.909
@2matrix
.04123 -.39379 -.91827 .64334 .71364 -.27716 .76448 -.57934 .28276
@group {myoglobin} 
@subgroup dominant {cap Hb}
@vectorlist {cap box Hb} color= purple 
{og ser 3}P U 6.003 10.194 19.797 {n glu 6}U 7.66 10.491 19.57
{oe2 glu 6}P U 4.66 11.241 16.95 {n ser 3}U 4.344 12.159 18.106
@balllist {cap O} color= red radius= .2 master= {polar}
{og ser 3}5.32 10.072 19.891
{oe2 glu 6}4.807 10.816 16.414
@balllist {cap N} color= sky radius= .2
{n ser 3}4.197 12.584 18.642
{n glu 6}8.343 10.613 19.476
@subgroup dominant {main ch}
@vectorlist {mc} color= white 
{ca val 1}P 0.621 15.543 16.081 {c val 1}1.823 14.549 16.2
{o val 1}1.628 13.369 15.953
{c val 1}P 1.823 14.549 16.2 {n leu 2}3.112 14.944 16.272
{ca leu 2}4.14 13.989 16.663
{c leu 2}3.978 13.813 18.182
{o leu 2}3.77 14.833 18.864
{c leu 2}P 3.978 13.813 18.182 {n ser 3}4.197 12.584 18.642
{ca ser 3}4.299 12.314 20.105
{c ser 3}5.679 12.782 20.606
{o ser 3}6.574 13.117 19.827
{c ser 3}P 5.679 12.782 20.606 {n glu 4}5.725 12.947 21.891
{ca glu 4}6.922 13.372 22.546
{c glu 4}8.05 12.356 22.255
{o glu 4}9.149 12.827 21.934
{c glu 4}P 8.05 12.356 22.255 {n gly 5}7.74 11.03 22.158
{ca gly 5}8.726 10.011 21.812
{c gly 5}9.27 10.178 20.38
{o gly 5}10.483 10.08 20.163
{c gly 5}P 9.27 10.178 20.38 {n glu 6}8.343 10.613 19.476
{ca glu 6}8.8 10.856 18.079
{c glu 6}9.687 12.097 18.053
{o glu 6}10.628 12.148 17.262
{c glu 6}P 9.687 12.097 18.053 {n trp 7}9.304 13.123 18.802
{ca trp 7}10.155 14.314 18.776
{c trp 7}11.562 14.011 19.286
{o trp 7}12.527 14.571 18.765
{c trp 7}P 11.562 14.011 19.286 {n gln 8}11.614 13.129 20.274
{ca gln 8}12.922 12.849 20.914
{c gln 8}13.848 12.102 19.935
{o gln 8}15.007 12.424 19.772
{c gln 8}P 13.848 12.102 19.935 {n leu 9}13.27 11.272 19.089
{ca leu 9}13.949 10.568 17.972
{c leu 9}14.499 11.595 16.988
{o leu 9}15.618 11.425 16.514
{c leu 9}P 14.499 11.595 16.988 {n val 10}13.687 12.549 16.601
{ca val 10}14.103 13.599 15.615
{c val 10}15.231 14.448 16.171
{o val 10}16.23 14.67 15.48
{c val 10}P 15.231 14.448 16.171 {n leu 11}15.087 14.852 17.404
{ca leu 11}16.064 15.786 17.964
{c leu 11}17.348 15.109 18.371
{o leu 11}18.425 15.734 18.236
{c leu 11}P 17.348 15.109 18.371 {n his 12}17.26 13.806 18.568
{ca his 12}18.511 13.098 18.904
{c his 12}19.385 13.061 17.663
{o his 12}20.613 13.301 17.785
{c his 12}P 19.385 13.061 17.663 {n val 13}18.747 12.659 16.538
{ca val 13}19.562 12.593 15.317
{c val 13}19.985 14.035 14.92
{o val 13}21.115 14.277 14.457
{c val 13}P 19.985 14.035 14.92 {n trp 14}19.134 15.032 15.075
{ca trp 14}19.498 16.373 14.667
{c trp 14}20.748 16.919 15.451
{o trp 14}21.562 17.639 14.864
{c trp 14}P 20.748 16.919 15.451 {n ala 15}20.883 16.545 16.709
{ca ala 15}22.011 17.011 17.558
@vectorlist {hlx Hb} color= brown master= {helix Hb} 
<1.011r= >{trp 7 hn} P U 8.472 13.078 19.356 {ser 3 o} U 7.143 13.105 19.686
<2.011r= >{gln 8 hn} P U 10.776 12.67 20.569 {glu 4 o} U 9.637 12.78 21.524
<2.011r= >{leu 9 hn} P U 12.292 11.12 19.232 {gly 5 o} U 11.026 10.392 19.884
<2.011r= >{val 10 hn} P U 12.769 12.545 16.997 {glu 6 o} U 11.27 12.267 17.182
<1.011r= >{leu 11 hn} P U 14.314 14.517 17.942 {trp 7 o} U 13.063 14.555 18.518
<2.011r= >{his 12 hn} P U 16.38 13.337 18.489 {gln 8 o} U 15.419 12.698 19.387
<2.011r= >{val 13 hn} P U 17.776 12.419 16.532 {leu 9 o} U 16.265 11.723 16.519
<2.011r= >{trp 14 hn} P U 18.235 14.858 15.476 {val 10 o} U 16.831 14.726 15.479
<2.011r= >{ala 15 hn} P U 20.191 15.927 17.082 {leu 11 o} U 18.955 15.792 17.89
@subgroup dominant {side ch} 
@vectorlist {Hphobic} color= gold master= {Hphobic}
{ca leu 2}P 4.14 13.989 16.663 {cb leu 2}5.553 14.489 16.342
{cg leu 2}6.119 14.183 14.949
{cd1 leu 2}5.542 15.081 13.861
{cg leu 2}P 6.119 14.183 14.949 {cd2 leu 2}7.651 14.312 14.995
{ca trp 7}P 10.155 14.314 18.776 {cb trp 7}9.627 15.506 19.524
{cg trp 7}8.499 16.238 18.786
{cd1 trp 7}7.2 16.402 19.218
{ne1 trp 7}6.518 17.169 18.273
{ce2 trp 7}7.355 17.495 17.233
{cg trp 7}P 8.499 16.238 18.786 {cd2 trp 7}8.623 16.957 17.54
{ce2 trp 7}7.355 17.495 17.233
{cz2 trp 7}7.081 18.226 16.085
{ch2 trp 7}8.14 18.427 15.202
{cd2 trp 7}P 8.623 16.957 17.54 {ce3 trp 7}9.69 17.169 16.67
{cz3 trp 7}9.435 17.909 15.494
{ch2 trp 7}8.14 18.427 15.202
@vectorlist {polar cap} color= sea master= {polar} 
{ca ser 3}P 4.299 12.314 20.105 {cb ser 3}4.192 10.803 20.419
{og ser 3}5.32 10.072 19.891
{ca glu 6}P 8.8 10.856 18.079 {cb glu 6}7.598 10.989 17.142
{cg glu 6}6.878 9.638 16.908
{cd glu 6}5.421 9.759 16.394
{oe1 glu 6}4.99 8.844 15.709
{cd glu 6}P 5.421 9.759 16.394 {oe2 glu 6}4.807 10.816 16.414
@group {Trp repr} 
@subgroup dominant {box Hb}
@vectorlist {hb} color= purple
{og1 thr r 44}P U 6.054 10.283 19.635 {n glu r 47}U 7.709 10.507 19.55
{oe1 glu r 47}P U 4.785 11.238 16.931 {n thr r 44}U 4.431 12.177 18.067
{nh1 arg r 48}P U 4.728 16.112 19.904 {o leu r 43}U 3.87 15.141 19.245
@balllist {O} color= red radius= .2 master= {polar}
{og1 thr r 44}5.361 10.19 19.671
{oe1 glu r 47}4.948 10.804 16.407
@balllist {N} color= sky radius= .2
{n thr r 44}4.268 12.611 18.591
{n glu r 47}8.402 10.6 19.514
@subgroup dominant {main ch} 
@vectorlist {mc} color= white 
{ca met r 42}P 1.23 15.95 14.905 {c met r 42}2.139 14.837 15.378
{o met r 42}1.929 13.701 14.917
{c met r 42}P 2.139 14.837 15.378 {n leu r 43}3.122 15.142 16.224
{ca leu r 43}4.026 14.067 16.678
{c leu r 43}3.872 13.796 18.172
{o leu r 43}3.456 14.674 18.928
{c leu r 43}P 3.872 13.796 18.172 {n thr r 44}4.268 12.611 18.591
{ca thr r 44}4.234 12.311 20.047
{c thr r 44}5.574 12.772 20.63
{o thr r 44}6.55 12.962 19.904
{c thr r 44}P 5.574 12.772 20.63 {n pro r 45}5.651 12.937 21.95
{ca pro r 45}6.895 13.328 22.607
{c pro r 45}8.019 12.35 22.223
{o pro r 45}9.155 12.798 21.958
{c pro r 45}P 8.019 12.35 22.223 {n asp r 46}7.739 11.046 22.193
{ca asp r 46}8.776 10.053 21.836
{c asp r 46}9.286 10.211 20.404
{o asp r 46}10.475 9.949 20.143
{c asp r 46}P 9.286 10.211 20.404 {n glu r 47}8.402 10.6 19.514
{ca glu r 47}8.813 10.785 18.099
{c glu r 47}9.706 12.007 18.007
{o glu r 47}10.708 12.053 17.262
{c glu r 47}P 9.706 12.007 18.007 {n arg r 48}9.338 13.067 18.738
{ca arg r 48}10.167 14.298 18.727
{c arg r 48}11.57 13.977 19.273
{o arg r 48}12.566 14.408 18.657
{c arg r 48}P 11.57 13.977 19.273 {n glu r 49}11.683 13.234 20.353
{ca glu r 49}12.959 12.819 20.921
{c glu r 49}13.736 11.992 19.89
{o glu r 49}14.953 12.271 19.689
{c glu r 49}P 13.736 11.992 19.89 {n ala r 50}13.065 11.044 19.249
{ca ala r 50}13.734 10.205 18.23
{c ala r 50}14.3 11.066 17.109
{o ala r 50}15.447 10.859 16.669
{c ala r 50}P 14.3 11.066 17.109 {n leu r 51}13.541 12.053 16.614
{ca leu r 51}14.053 12.898 15.525
{c leu r 51}15.281 13.706 15.963
{o leu r 51}16.196 13.863 15.152
{c leu r 51}P 15.281 13.706 15.963 {n gly r 52}15.262 14.198 17.199
{ca gly r 52}16.436 14.961 17.674
{c gly r 52}17.658 14.028 17.683
{o gly r 52}18.767 14.477 17.297
{c gly r 52}P 17.658 14.028 17.683 {n thr r 53}17.478 12.793 18.103
{ca thr r 53}18.598 11.812 18.148
{c thr r 53}19.096 11.442 16.767
{o thr r 53}20.309 11.328 16.507
{c thr r 53}P 19.096 11.442 16.767 {n arg r 54}18.124 11.293 15.849
{ca arg r 54}18.49 10.94 14.45
{c arg r 54}19.351 12.021 13.827
{o arg r 54}20.273 11.684 13.045
{c arg r 54}P 19.351 12.021 13.827 {n val r 55}19.079 13.304 14.082
{ca val r 55}19.935 14.349 13.512
{c val r 55}21.389 14.188 14.016
{o val r 55}22.318 14.328 13.206
{c val r 55}P 21.389 14.188 14.016 {n arg r 56}21.508 13.901 15.298
{ca arg r 56}22.856 13.688 15.882
@vectorlist {hlx Hb} color= brown master= {helix Hb}
<2.050>{arg 48 hn} P U 8.504 13.019 19.287 {thr 44 o} U 7.136 12.979 19.719
<2.047>{glu 49 hn} P U 10.839 12.942 20.803 {pro 45 o} U 9.66 12.841 21.611
<2.003>{ala 50 hn} P U 12.099 10.907 19.468 {asp 46 o} U 10.962 10.236 19.941
<1.945>{leu 51 hn} P U 12.627 12.213 16.987 {glu 47 o} U 11.284 12.101 17.179
<2.121>{gly 52 hn} P U 14.465 14.048 17.784 {arg 48 o} U 13.136 14.3 18.395
<2.079>{thr 53 hn} P U 16.557 12.534 18.393 {glu 49 o} U 15.434 12.35 19.3
<1.894>{arg 54 hn} P U 17.168 11.42 16.113 {ala 50 o} U 15.963 11.027 16.502
<2.182>{val 55 hn} P U 18.298 13.547 14.657 {leu 51 o} U 16.827 13.768 15.003
<2.481>{arg 56 hn} P U 20.68 13.832 15.855 {gly 52 o} U 19.341 14.283 16.864
@subgroup dominant {side ch} 
@vectorlist {Hphobic} color= gold master= {Hphobic}
{ca leu r 43}P 4.026 14.067 16.678 {cb leu r 43}5.461 14.614 16.428
{cg leu r 43}5.853 14.751 14.946
{cd1 leu r 43}7.203 15.488 14.846
{cg leu r 43}P 5.853 14.751 14.946 {cd2 leu r 43}5.919 13.367 14.324
{ca arg r 48}P 10.167 14.298 18.727 {cb arg r 48}9.517 15.469 19.45
{cg arg r 48}8.171 15.866 18.818
{cd arg r 48}7.732 17.2 19.357
{ne arg r 48}7.338 17.118 20.767
@vectorlist {polar} color= sea master= {polar}
{ca thr r 44}P 4.234 12.311 20.047 {cb thr r 44}4.126 10.758 20.267
{og1 thr r 44}5.361 10.19 19.671
{cb thr r 44}P 4.126 10.758 20.267 {cg2 thr r 44}2.928 10.122 19.58
{ca glu r 47}P 8.813 10.785 18.099 {cb glu r 47}7.59 10.881 17.208
{cg glu r 47}6.874 9.534 16.948
{cd glu r 47}5.435 9.696 16.515
{oe1 glu r 47}4.948 10.804 16.407
{cd glu r 47}P 5.435 9.696 16.515 {oe2 glu r 47}4.832 8.596 16.301
{ne arg r 48}P 7.338 17.118 20.767 {cz arg r 48}6.083 16.838 21.137
{nh1 arg r 48}5.142 16.579 20.221
{cz arg r 48}P 6.083 16.838 21.137 {nh2 arg r 48}5.732 16.876 22.429
@group {carb anhydr} 
@subgroup dominant {box Hb}
@vectorlist {hb} color= purple
{og ser 219}P U 6.025 10.25 19.687 {n gln 222}U 7.82 10.494 19.574
{oe1 gln 222}P U 4.913 11.142 16.54 {n ser 219}U 4.33 12.217 18.033
@balllist {O} color= red radius= .2 master= {polar}
{og ser 219}5.333 10.155 19.731
{oe1 gln 222}5.125 10.752 15.998
@balllist {N} color= sky radius= .2
{n ser 219}4.118 12.607 18.575
{n gln 222}8.512 10.589 19.53
@subgroup dominant {main ch} 
@vectorlist {mc} color= white
{ca ser 217}P 1.05 16.688 15.832 {c ser 217}1.833 15.389 15.732
{o ser 217}1.609 14.505 14.855
{c ser 217}P 1.833 15.389 15.732 {n val 218}2.805 15.293 16.593
{ca val 218}3.7 14.135 16.696
{c val 218}3.83 13.821 18.193
{o val 218}3.691 14.768 18.96
{c val 218}P 3.83 13.821 18.193 {n ser 219}4.118 12.607 18.575
{ca ser 219}4.278 12.314 19.996
{c ser 219}5.637 12.803 20.501
{o ser 219}6.669 13.04 19.785
{c ser 219}P 5.637 12.803 20.501 {n ser 220}5.639 12.903 21.793
{ca ser 220}6.798 13.3 22.582
{c ser 220}7.968 12.354 22.299
{o ser 220}9.111 12.852 22.133
{c ser 220}P 7.968 12.354 22.299 {n glu 221}7.72 11.044 22.195
{ca glu 221}8.709 10.017 21.902
{c glu 221}9.318 10.116 20.504
{o glu 221}10.507 9.774 20.29
{c glu 221}P 9.318 10.116 20.504 {n gln 222}8.512 10.589 19.53
{ca gln 222}8.964 10.801 18.149
{c gln 222}9.953 11.959 18.087
{o gln 222}11.091 11.79 17.583
{c gln 222}P 9.953 11.959 18.087 {n val 223}9.576 13.117 18.669
{ca val 223}10.504 14.288 18.668
{c val 223}11.741 13.944 19.502
{o val 223}12.809 14.444 19.195
{c val 223}P 11.741 13.944 19.502 {n leu 224}11.594 13.112 20.578
{ca leu 224}12.685 12.683 21.399
{c leu 224}13.843 12.026 20.633
{o leu 224}15.035 12.348 20.931
{c leu 224}P 13.843 12.026 20.633 {n lys 225}13.507 11.202 19.707
{ca lys 225}14.39 10.495 18.772
{c lys 225}15.162 11.435 17.905
{o lys 225}16.352 11.201 17.64
{c lys 225}P 15.162 11.435 17.905 {n phe 226}14.528 12.543 17.446
{ca phe 226}15.274 13.537 16.633
{c phe 226}16.437 14.115 17.476
{o phe 226}17.487 14.377 16.926
{c phe 226}P 16.437 14.115 17.476 {n arg 227}16.247 14.341 18.739
{ca arg 227}17.228 14.926 19.68
{c arg 227}18.435 14.096 20.061
{o arg 227}19.351 14.562 20.751
{c arg 227}P 18.435 14.096 20.061 {n lys 228}18.448 12.842 19.638
{ca lys 228}19.453 11.827 19.839
{c lys 228}20.495 11.841 18.729
{o lys 228}21.546 11.211 18.911
{c lys 228}P 20.495 11.841 18.729 {n leu 229}20.264 12.526 17.666
{ca leu 229}21.145 12.701 16.521
@vectorlist {hb} color= brown master= {helix Hb}
<2.125>{val 223 hn} P U 8.675 13.182 19.098 {ser 219 o} U 7.271 13.083 19.579
<2.047>{leu 224 hn} P U 10.692 12.765 20.835 {ser 220 o} U 9.585 12.826 21.744
<2.469>{lys 225 hn} P U 12.521 11.049 19.645 {glu 221 o} U 11.111 10.156 20.097
<2.630>{phe 226 hn} P U 13.561 12.69 17.655 {gln 222 o} U 11.832 12.06 17.605
<2.560>{arg 227 hn} P U 15.341 14.084 19.075 {val 223 o} U 13.569 14.336 19.159
<2.381>{lys 228 hn} P U 17.652 12.555 19.105 {lys 225 o} U 16.742 11.607 18.08
<2.457>{leu 229 hn} P U 19.372 12.978 17.652 {phe 226 o} U 18.052 13.957 17.144
@subgroup dominant {side ch} 
@vectorlist {Hphobic} color= gold master= {Hphobic}
{ca val 218}P 3.7 14.135 16.696 {cb val 218}5.021 14.372 15.956
{cg1 val 218}4.705 14.581 14.46
{cb val 218}P 5.021 14.372 15.956 {cg2 val 218}5.841 15.51 16.483
{ca val 223}P 10.504 14.288 18.668 {cb val 223}9.865 15.624 19.061
{cg1 val 223}10.909 16.69 19.444
{cb val 223}P 9.865 15.624 19.061 {cg2 val 223}9.022 16.285 17.948
@vectorlist {polar} color= sea master= {polar}
{ca ser 219}P 4.278 12.314 19.996 {cb ser 219}4.229 10.808 20.284
{og ser 219}5.333 10.155 19.731
{ca gln 222}P 8.964 10.801 18.149 {cb gln 222}7.726 11.058 17.226
{cg gln 222}7.152 9.762 16.723
{cd gln 222}5.921 9.844 15.883
{oe1 gln 222}5.125 10.752 15.998
{cd gln 222}P 5.921 9.844 15.883 {ne2 gln 222}5.705 8.865 14.969
@kinemage 2
@caption
Cytochrome C,  alpha helix with Asn capping box.  Hydrophobic sidechains are in gold, polar ones in seagreen.
@onewidth 
@viewid {cap box}
@zoom 0.95
@zslab 140
@center 10.603 -5.111 12.988
@matrix
-.53549 -.07042 .8416 .59364 .67741 .4344 -.6007 .73223 -.32094
@2viewid {helix end}
@2zoom 1.05
@2zslab 140 
@2center 10.660 -3.362 12.688 
@2matrix 
-.91923  .39371 .0035 -.26105 -.61609 .74316 .29475 .68222 .6691
@group {Cyt C} 
@subgroup dominant {box Hb}
@vectorlist {hb} color= purple
{od1 asn r 60}P U 10.275 -0.249 14.025 {n thr r 63}U 10.297 -1.2 12.791
{og1 thr r 63}P U 11.632 -2.596 14.849 {n asn r 60}U 10.812 -2.343 16.18
@balllist {box O} color= red radius= .2 master= {polar}
{od1 asn r 60}10.265 0.178 14.579
{og1 thr r 63}11.994 -2.707 14.261
@balllist {box N} color= sky radius= .2
{n asn r 60}10.45 -2.232 16.768
{n thr r 63}10.307 -1.627 12.237
@subgroup dominant {main ch} 
@vectorlist {mc} color= white 
{ca val r 58}P 15.538 -1.466 17.757 {c val r 58}14.193 -1.985 17.24
{o val r 58}13.798 -1.663 16.115
{c val r 58}P 14.193 -1.985 17.24 {n trp r 59}13.62 -2.943 17.96
{ca trp r 59}12.33 -3.522 17.533
{c trp r 59}11.168 -2.568 17.812
{o trp r 59}10.989 -2.082 18.929
{c trp r 59}P 11.168 -2.568 17.812 {n asn r 60}10.45 -2.232 16.768
{ca asn r 60}9.282 -1.342 16.894
{c asn r 60}8.36 -1.647 15.71
{o asn r 60}8.615 -2.608 14.979
{c asn r 60}P 8.36 -1.647 15.71 {n asn r 61}7.242 -0.92 15.589
{ca asn r 61}6.272 -1.305 14.539
{c asn r 61}6.944 -1.367 13.172
{o asn r 61}6.74 -2.289 12.385
{c asn r 61}P 6.944 -1.367 13.172 {n asp r 62}7.795 -0.389 12.948
{ca asp r 62}8.415 -0.236 11.641
{c asp r 62}9.397 -1.372 11.323
{o asp r 62}9.334 -1.983 10.25
{c asp r 62}P 9.397 -1.372 11.323 {n thr r 63}10.307 -1.627 12.237
{ca thr r 63}11.275 -2.711 11.958
{c thr r 63}10.656 -4.105 12.019
{o thr r 63}11.014 -4.97 11.212
{c thr r 63}P 10.656 -4.105 12.019 {n leu r 64}9.643 -4.268 12.862
{ca leu r 64}8.92 -5.572 12.815
{c leu r 64}8.196 -5.822 11.485
{o leu r 64}8.154 -6.946 10.985
{c leu r 64}P 8.196 -5.822 11.485 {n met r 65}7.577 -4.794 10.949
{ca met r 65}6.854 -4.963 9.686
{c met r 65}7.819 -5.36 8.576
{o met r 65}7.491 -6.173 7.707
{c met r 65}P 7.819 -5.36 8.576 {n glu r 66}9.011 -4.797 8.637
{ca glu r 66}10.02 -5.151 7.645
{c glu r 66}10.572 -6.572 7.836
{o glu r 66}10.639 -7.361 6.889
{c glu r 66}P 10.572 -6.572 7.836 {n tyr r 67}10.918 -6.866 9.078
{ca tyr r 67}11.488 -8.186 9.422
{c tyr r 67}10.51 -9.302 9.063
{o tyr r 67}10.879 -10.262 8.398
{c tyr r 67}P 10.51 -9.302 9.063 {n leu r 68}9.281 -9.135 9.484
{ca leu r 68}8.293 -10.209 9.308
{c leu r 68}7.887 -10.473 7.863
{o leu r 68}7.437 -11.584 7.586
{c leu r 68}P 7.887 -10.473 7.863 {n glu r 69}8.221 -9.578 6.961
{ca glu r 69}7.96 -9.878 5.541
{c glu r 69}8.926 -10.945 5.04
{o glu r 69}8.55 -11.849 4.285
{c glu r 69}P 8.926 -10.945 5.04 {n asn r 70}10.169 -10.833 5.491
{ca asn r 70}11.172 -11.796 5.006
@vectorlist {hlx Hb} color= brown master= {helix Hb}
<1.311r>{leu 64 hn} P U 9.392 -3.535 13.494 {asn 60 o} U 8.848 -2.886 14.534
<2.311r>{met 65 hn} P U 7.608 -3.908 11.412 {asn 61 o} U 7. -2.775 12.093
<2.311r>{glu 66 hn} P U 9.212 -4.135 9.359 {asp 62 o} U 9.297 -2.629 9.983
<1.311r>{tyr 67 hn} P U 10.785 -6.171 9.785 {thr 63 o} U 10.945 -5.33 10.784
<1.311r>{leu 68 hn} P U 9.035 -8.27 9.922 {leu 64 o} U 8.418 -7.343 10.666
<2.311r>{glu 69 hn} P U 8.642 -8.719 7.252 {glu 66 o} U 10.04 -7.768 6.998
<2.311r>{asn 70 hn} P U 10.406 -10.111 6.141 {glu 66 o} U 10.569 -8.186 6.665
<2.311r>{asn 70 hn} P U 10.406 -10.111 6.141 {tyr 67 o} U 10.737 -10.217 7.721
@subgroup dominant {side ch} 
@vectorlist {Hphobic} color= gold master= {Hphobic}
{ca trp r 59}P 12.33 -3.522 17.533 {cb trp r 59}12.066 -4.859 18.215
{cg trp r 59}12.973 -5.983 17.747
{cd1 trp r 59}13.785 -6.777 18.537
{ne1 trp r 59}14.41 -7.679 17.759
{ce2 trp r 59}14.063 -7.533 16.529
{cg trp r 59}P 12.973 -5.983 17.747 {cd2 trp r 59}13.144 -6.465 16.447
{ce2 trp r 59}14.063 -7.533 16.529
{cz2 trp r 59}14.477 -8.267 15.418
{ch2 trp r 59}13.927 -7.895 14.184
{cd2 trp r 59}P 13.144 -6.465 16.447 {ce3 trp r 59}12.601 -6.098 15.232
{cz3 trp r 59}13.002 -6.833 14.093
{ch2 trp r 59}13.927 -7.895 14.184
{ca leu r 64}P 8.92 -5.572 12.815 {cb leu r 64}7.932 -5.665 13.977
{cg leu r 64}8.605 -5.983 15.298
{cd1 leu r 64}7.705 -5.702 16.498
{cg leu r 64}P 8.605 -5.983 15.298 {cd2 leu r 64}9.111 -7.436 15.295
@vectorlist {polar cap} color= sea master= {polar} 
{ca asn r 60}P 9.282 -1.342 16.894 {cb asn r 60}9.709 0.154 16.951
{cg asn r 60}10.513 0.608 15.706
{od1 asn r 60}10.265 0.178 14.579
{cg asn r 60}P 10.513 0.608 15.706 {nd2 asn r 60}11.48 1.549 15.869
{ca thr r 63}P 11.275 -2.711 11.958 {cb thr r 63}12.457 -2.637 12.916
{og1 thr r 63}11.994 -2.707 14.261
{cb thr r 63}P 12.457 -2.637 12.916 {cg2 thr r 63}13.229 -1.346 12.707
@kinemage 3
@caption 
"Near" helix capping box, from human lysozyme.  Hydrophobic sidechains are in gold, polar ones in seagreen.
@onewidth 
@viewid {cap box}
@zoom 1.15
@zslab 110
@center 20.008 16.104 25.104
@matrix 
.005328 .637233 .770653 .481272 .673905 -.560562 -.876556 .37388 -.303091
@2viewid {helix end}
@2zoom 1.35 
@2zslab 140 
@2center 22.305 17.825 25.95
@2matrix 
-.598346 .059148 .799052 .724599 -.385681 .571144 .341961 .920734 .187913 
@group {lysozyme} 
@subgroup {box Hb} dominant 
@vectorlist {hb} color= purple master= {polar} 
{og ser 24}P U 24.793 20.226 24.321 {n asn 27}U 23.193 19.362 23.649
@balllist {O} color= red master= {polar} radius= 0.2
{og ser 24}25.371 20.538 24.564
@balllist {N} color= sky radius= 0.2
{nd2 asn 27}25.715 17.604 24.698
{n ser 24}24.947 20.679 27.492
{n asn 27}22.615 19.05 23.406
@subgroup {main ch} dominant 
@vectorlist {mc} color= white 
{ca gly 22}P 27.243 20.412 32.34 {c gly 22}26.757 19.972 30.957
{o gly 22}27.51 19.689 30.005
{c gly 22}P 26.757 19.972 30.957 {n ile 23}25.417 19.985 30.8
{ca ile 23}24.82 19.481 29.569
{c ile 23}24.393 20.649 28.696
{o ile 23}23.543 21.526 29.005
{c ile 23}P 24.393 20.649 28.696 {n ser 24}24.947 20.679 27.492
{ca ser 24}24.698 21.791 26.548
{c ser 24}23.25 21.718 26.06
{o ser 24}22.607 20.632 25.943
{c ser 24}P 23.25 21.718 26.06 {n leu 25}22.661 22.845 25.627
{ca leu 25}21.356 22.886 24.94
{c leu 25}21.27 21.931 23.741
{o leu 25}20.235 21.217 23.612
{c leu 25}P 21.27 21.931 23.741 {n ala 26}22.353 21.762 22.941
{ca ala 26}22.265 20.881 21.777
{c ala 26}22.07 19.434 22.229
{o ala 26}21.346 18.667 21.538
{c ala 26}P 22.07 19.434 22.229 {n asn 27}22.615 19.05 23.406
{ca asn 27}22.382 17.655 23.877
{c asn 27}20.91 17.519 24.285
{o asn 27}20.343 16.439 24.098
{c asn 27}P 20.91 17.519 24.285 {n trp 28}20.33 18.481 24.976
{ca trp 28}18.913 18.388 25.39
{c trp 28}18.069 18.303 24.123
{o trp 28}17.028 17.598 24.108
{c trp 28}P 18.069 18.303 24.123 {n met 29}18.372 19.089 23.098
{ca met 29}17.595 19.088 21.845
{c met 29}17.698 17.707 21.187
{o met 29}16.669 17.163 20.695
{c met 29}P 17.698 17.707 21.187 {n cys 30}18.912 17.131 21.175
{ca cys 30}19.017 15.799 20.555
{c cys 30}18.161 14.834 21.362
{o cys 30}17.495 13.956 20.74
{c cys 30}P 18.161 14.834 21.362 {n leu 31}18.127 14.943 22.682
{ca leu 31}17.374 13.994 23.504
{c leu 31}15.899 14.134 23.148
{o leu 31}15.198 13.166 22.826
{c leu 31}P 15.899 14.134 23.148 {n ala 32}15.358 15.37 23.168
{ca ala 32}13.934 15.574 22.851
{c ala 32}13.616 15.108 21.435
{o ala 32}12.53 14.557 21.142
{c ala 32}P 13.616 15.108 21.435 {n lys 33}14.479 15.348 20.451
{ca lys 33}14.178 14.932 19.068
{c lys 33}13.936 13.412 19.031
{o lys 33}12.914 12.991 18.397
{c lys 33}P 13.936 13.412 19.031 {n trp 34}14.91 12.681 19.587
{ca trp 34}14.812 11.223 19.366
{c trp 34}13.808 10.646 20.354
{o trp 34}13.194 9.576 20.098
{c trp 34}P 13.808 10.646 20.354 {n glu 35}13.526 11.283 21.489
{ca glu 35}12.529 10.678 22.385
{c glu 35}11.087 10.839 21.897
{o glu 35}10.322 9.868 22.015
{c glu 35}P 11.087 10.839 21.897 {n ser 36}10.759 12.055 21.458
{ca ser 36}9.333 12.354 21.192
{c ser 36}9.135 13.032 19.846
{o ser 36}7.968 13.358 19.499
{c ser 36}P 9.135 13.032 19.846 {n gly 37}10.172 13.415 19.098
{ca gly 37}9.951 14.264 17.909
{c gly 37}9.476 15.649 18.324
{o gly 37}8.765 16.32 17.547
{c gly 37}P 9.476 15.649 18.324 {n tyr 38}9.813 16.107 19.532
{ca tyr 38}9.43 17.416 20.065
@vectorlist {hb} color= brown master= {helix Hb} 
<2.517r>{trp 28 hn} P U 20.878 19.283 25.215 {ser 24 o} U 22.088 20.227 25.725
<1.517r>{met 29 hn} P U 19.158 19.7 23.191 {leu 25 o} U 19.912 20.762 23.486
<1.517r>{cys 30 hn} P U 19.709 17.588 21.57 {ala 26 o} U 20.855 18.343 21.548
<2.517r>{leu 31 hn} P U 18.628 15.691 23.118 {asn 27 o} U 19.829 16.215 23.804
<1.517r>{ala 32 hn} P U 15.929 16.158 23.4 {trp 28 o} U 16.698 17.166 23.896
<1.517r>{lys 33 hn} P U 15.337 15.816 20.662 {met 29 o} U 16.269 16.759 20.685
<2.517r>{trp 34 hn} P U 15.65 13.099 20.114 {cys 30 o} U 16.941 13.699 20.552
<1.517r>{glu 35 hn} P U 13.981 12.144 21.718 {leu 31 o} U 14.833 12.859 22.494
<2.517r>{ser 36 hn} P U 11.471 12.743 21.315 {ala 32 o} U 12.212 14.013 21.194
<2.517r>{gly 37 hn} P U 11.094 13.122 19.35 {lys 33 o} U 12.368 13.03 18.683
<2.517r>{tyr 38 hn} P U 10.367 15.511 20.114 {ala 32 o} U 11.881 14.843 20.834
@subgroup {side ch} dominant 
@vectorlist {sc} color= gold master= {Hphobic} 
{ca ile 23}P 24.82 19.481 29.569 {cb ile 23}23.524 18.7 29.889
{cg1 ile 23}23.867 17.524 30.843
{cd1 ile 23}22.617 16.913 31.537
{cb ile 23}P 23.524 18.7 29.889 {cg2 ile 23}22.799 18.176 28.649
{ca trp 28}P 18.913 18.388 25.39 {cb trp 28}18.494 19.627 26.168
{cg trp 28}19.116 19.672 27.611
{cd1 trp 28}19.95 20.634 28.107
{ne1 trp 28}20.165 20.292 29.482
{ce2 trp 28}19.489 19.231 29.823
{cg trp 28}P 19.116 19.672 27.611 {cd2 trp 28}18.764 18.811 28.656
{ce2 trp 28}19.489 19.231 29.823
{cz2 trp 28}19.341 18.634 31.082
{ch2 trp 28}18.487 17.567 31.065
{cd2 trp 28}P 18.764 18.811 28.656 {ce3 trp 28}17.932 17.717 28.706
{cz3 trp 28}17.778 17.055 29.96
{ch2 trp 28}18.487 17.567 31.065
@vectorlist {sc} color= sea master= {polar} 
{ca ser 24}P 24.698 21.791 26.548 {cb ser 24}25.564 21.73 25.307
{og ser 24}25.371 20.538 24.564
{ca asn 27}P 22.382 17.655 23.877 {cb asn 27}23.375 17.358 25.034
{cg asn 27}24.643 16.769 24.451
{od1 asn 27}24.659 15.721 23.812
{cg asn 27}P 24.643 16.769 24.451 {nd2 asn 27}25.715 17.604 24.698
@kinemage 4
@caption
Myoglobin and DHFR - superimposed helices with "big" capping boxes.  Hydrophobic sidechains are in gold, polar ones in seagreen.
@onewidth 
@viewid {cap box}
@zoom 1.0
@zslab 120
@center 31.063 22.457 -8.783
@matrix
.656147 -.200817 .727423 -.675219 -.58667 .447099 .336972 -.784532 -.520537
@2viewid {helix end}
@2zoom 1.0
@2zslab 140
@2center 30.396 21.827 -8.972
@2matrix
-.713286 -.700133 -.032201 -.564118 .600768 -.566435 .415925 -.385865 -0.823477 
@group {myoglobin} 
@subgroup {box Hb} dominant 
@vectorlist {hb} color= purple 
{n lys 50}P U 28.912 22.658 -12.727 {oe2 glu 54}U 30.278 22.679 -12.762
{og1 thr 51}P U 32.13 19.425 -11.136 {n glu 54}U 32.458 20.011 -9.63
@balllist {cap O} color= red radius= 0.2 master= {polar}
{og1 thr 51}31.99 19.176 -11.775
{oe2 glu 54}30.978 22.689 -12.78
@balllist {cap N} color= sky radius= 0.2
{n lys 50}28.212 22.648 -12.709
{n glu 54}32.598 20.26 -8.991
@subgroup dominant {main ch} 
@vectorlist {mc} color= white 
{n leu 49}P 27.1 25.805 -11.757 {ca leu 49}27.876 24.557 -11.352
{c leu 49}27.324 23.407 -12.159
{o leu 49}26.11 23.298 -12.294
{c leu 49}P 27.324 23.407 -12.159 {n lys 50}28.212 22.648 -12.709
{ca lys 50}27.691 21.58 -13.604
{c lys 50}28.035 20.173 -13.076
{o lys 50}27.157 19.296 -13.036
{c lys 50}P 28.035 20.173 -13.076 {n thr 51}29.185 20.025 -12.493
{ca thr 51}29.61 18.709 -12.036
{c thr 51}29.568 18.592 -10.533
{o thr 51}29.525 19.589 -9.825
{c thr 51}P 29.568 18.592 -10.533 {n glu 52}29.494 17.374 -10.065
{ca glu 52}29.513 17.144 -8.62
{c glu 52}30.788 17.718 -7.937
{o glu 52}30.688 18.292 -6.841
{c glu 52}P 30.788 17.718 -7.937 {n ala 53}31.964 17.531 -8.589
{ca ala 53}33.223 18.102 -8.037
{c ala 53}33.122 19.6 -7.962
{o ala 53}33.584 20.126 -6.965
{c ala 53}P 33.122 19.6 -7.962 {n glu 54}32.598 20.26 -8.991
{ca glu 54}32.441 21.706 -8.913
{c glu 54}31.513 22.077 -7.753
{o glu 54}31.813 22.982 -6.97
{c glu 54}P 31.513 22.077 -7.753 {n met 55}30.384 21.315 -7.576
{ca met 55}29.566 21.618 -6.428
{c met 55}30.259 21.497 -5.079
{o met 55}30.086 22.268 -4.135
{c met 55}P 30.259 21.497 -5.079 {n lys 56}31.019 20.402 -4.936
{ca lys 56}31.782 20.175 -3.701
{c lys 56}32.838 21.238 -3.404
{o lys 56}33.152 21.429 -2.227
{c lys 56}P 32.838 21.238 -3.404 {n ala 57}33.425 21.832 -4.471
{ca ala 57}34.457 22.835 -4.257
{c ala 57}33.891 24.198 -3.906
{o ala 57}34.667 25.075 -3.576
{c ala 57}P 33.891 24.198 -3.906 {n ser 58}32.564 24.447 -4.123
{ca ser 58}32.06 25.807 -3.905
{c ser 58}31.819 26.111 -2.42
{o ser 58}31.04 25.475 -1.687
{c ser 58}P 31.819 26.111 -2.42 {n glu 59}32.546 27.152 -2.023
{ca glu 59}32.422 27.614 -0.645
@vectorlist {hlx Hb} color= brown master= {helix Hb}
<1.969r>{met 55 hn} P U 30.113 20.578 -8.195 {thr 51 o} U 29.701 19.886 -9.336
<1.969r>{lys 56 hn} P U 31.066 19.734 -5.678 {glu 52 o} U 30.801 18.725 -6.492
<2.969r>{ala 57 hn} P U 33.161 21.593 -5.406 {ala 53 o} U 33.457 20.566 -6.497
<2.969r>{ser 58 hn} P U 31.937 23.728 -4.424 {met 55 o} U 30.641 22.706 -4.222
@subgroup dominant {side ch} 
@vectorlist {Hphobic} color= gold master= {Hphobic}
{ca leu 49}P 27.876 24.557 -11.352 {cb leu 49}27.584 24.173 -9.911
{cg leu 49}28.134 25.307 -9.014
{cd1 leu 49}27.576 25.238 -7.595
{cg leu 49}P 28.134 25.307 -9.014 {cd2 leu 49}29.689 25.34 -9.01
{ca met 55}P 29.566 21.618 -6.428 {cb met 55}28.367 20.632 -6.467
{cg met 55}27.47 21.043 -7.61
{sd met 55}26.095 19.82 -7.741
{ce met 55}25.56 20.191 -9.398
@vectorlist {polar cap} color= sea master= {polar} 
{ca thr 51}P 29.61 18.709 -12.036 {cb thr 51}30.985 18.43 -12.523
{og1 thr 51}31.99 19.176 -11.775
{cb thr 51}P 30.985 18.43 -12.523 {cg2 thr 51}31.125 18.654 -14.009
{ca glu 54}P 32.441 21.706 -8.913 {cb glu 54}31.87 22.3 -10.2
{cg glu 54}32.913 22.33 -11.287
{cd glu 54}32.235 22.589 -12.632
{oe1 glu 54}33.023 22.556 -13.579
{cd glu 54}P 32.235 22.589 -12.632 {oe2 glu 54}30.978 22.689 -12.78
@group {DHFR} 
@subgroup {box Hb} dominant 
@vectorlist {hb} color= purple
{n lys a 76}P U 28.987 22.978 -12.853 {oe2 glu a 80}U 30.707 23.529 -12.884
{og ser a 77}P U 32. 19.453 -11.212 {n glu a 80}U 32.426 20.018 -9.664
@balllist {cap O} color= red radius= 0.2 master= {polar}
{og ser a 77}31.825 19.221 -11.849
{oe2 glu a 80}31.564 23.804 -12.9
@balllist {cap N} color= sky radius= 0.2
{n lys a 76}28.13 22.703 -12.837
{n glu a 80}32.601 20.25 -9.027
@subgroup dominant {main ch} 
@vectorlist {mc} color= white 
{n val a 75}P 26.731 25.359 -10.97 {ca val a 75}27.734 24.375 -11.143
{c val a 75}27.157 23.334 -12.179
{o val a 75}26.009 23.106 -12.456
{c val a 75}P 27.157 23.334 -12.179 {n lys a 76}28.13 22.703 -12.837
{ca lys a 76}27.935 21.692 -13.932
{c lys a 76}28.352 20.403 -13.345
{o lys a 76}27.803 19.442 -14.022
{c lys a 76}P 28.352 20.403 -13.345 {n ser a 77}29.071 20.049 -12.352
{ca ser a 77}29.342 18.757 -11.896
{c ser a 77}29.47 18.612 -10.411
{o ser a 77}29.639 19.63 -9.706
{c ser a 77}P 29.47 18.612 -10.411 {n val a 78}29.47 17.441 -9.841
{ca val a 78}29.673 16.993 -8.545
{c val a 78}30.783 17.731 -7.849
{o val a 78}30.833 18.57 -6.746
{c val a 78}P 30.783 17.731 -7.849 {n asp a 79}31.929 17.451 -8.607
{ca asp a 79}33.109 18.11 -8.058
{c asp a 79}33.075 19.622 -8.036
{o asp a 79}33.574 19.873 -6.942
{c asp a 79}P 33.075 19.622 -8.036 {n glu a 80}32.601 20.25 -9.027
{ca glu a 80}32.607 21.683 -8.87
{c glu a 80}31.632 22.204 -7.776
{o glu a 80}31.829 23.239 -7.201
{c glu a 80}P 31.632 22.204 -7.776 {n ala a 81}30.589 21.454 -7.459
{ca ala a 81}29.599 21.761 -6.383
{c ala a 81}30.384 21.544 -5.113
{o ala a 81}30.258 22.572 -4.39
{c ala a 81}P 30.384 21.544 -5.113 {n ile a 82}31.102 20.579 -4.817
{ca ile a 82}31.884 20.373 -3.709
{c ile a 82}32.809 21.582 -3.364
{o ile a 82}32.925 22.261 -2.298
{c ile a 82}P 32.809 21.582 -3.364 {n ala a 83}33.534 21.933 -4.367
{ca ala a 83}34.528 23.03 -4.28
{c ala a 83}33.793 24.321 -4.048
{o ala a 83}34.391 25.02 -3.2
{c ala a 83}P 33.793 24.321 -4.048 {n ala a 84}32.68 24.613 -4.682
{ca ala a 84}31.917 25.838 -4.512
{c ala a 84}31.589 25.999 -3.049
{o ala a 84}31.279 27.155 -2.663
{c ala a 84}P 31.589 25.999 -3.049 {n cys a 85}31.587 25.017 -2.224
{ca cys a 85}31.243 25.061 -0.924
{c cys a 85}32.358 25.508 0.083
{o cys a 85}32.019 26.104 1.202
{c cys a 85}P 32.358 25.508 0.083 {n gly a 86}33.598 25.281 -0.418
{ca gly a 86}34.539 25.73 0.706
@vectorlist {hlx Hb} color= brown master= {helix Hb}
<2.969r>{ala 81 hn} P U 30.477 20.62 -7.999 {ser 77 o} U 29.89 19.927 -9.194
<1.969r>{ile 82 hn} P U 31.105 19.844 -5.495 {val 78 o} U 30.915 18.952 -6.371
<2.969r>{ala 83 hn} P U 33.39 21.423 -5.215 {asp 79 o} U 33.519 20.338 -6.424
<2.969r>{ala 84 hn} P U 32.338 23.933 -5.331 {glu 80 o} U 31.982 23.447 -6.64
<2.969r>{cys 85 hn} P U 31.864 24.111 -2.546 {ile 82 o} U 32.607 22.816 -2.372
<1.969r>{gly 86 hn} P U 33.822 24.906 -1.318 {ala 83 o} U 34.22 24.986 -2.635
@subgroup dominant {sidechain} 
@vectorlist {Hphobic} color= gold master= {Hphobic} 
{ca val a 75}P 27.734 24.375 -11.143 {cb val a 75}28.36 23.751 -9.988
{cg1 val a 75}29.137 24.716 -9.015
{cb val a 75}P 28.36 23.751 -9.988 {cg2 val a 75}27.473 22.866 -9.172
{ca ala a 81}P 29.599 21.761 -6.383 {cb ala a 81}28.441 20.708 -6.29
@vectorlist {polar} color= sea master= {polar}
{ca ser a 77}P 29.342 18.757 -11.896 {cb ser a 77}30.842 18.372 -12.453
{og ser a 77}31.825 19.221 -11.849
{ca glu a 80}P 32.607 21.683 -8.87 {cb glu a 80}31.815 22.056 -10.036
{cg glu a 80}32.579 22.944 -10.96
{cd glu a 80}32.155 22.744 -12.389
{oe1 glu a 80}32.379 21.607 -12.819
{cd glu a 80}P 32.155 22.744 -12.389 {oe2 glu a 80}31.564 23.804 -12.9