GroEL/GroES Mutational Database

Mutations in GroEL - Page 2

Residues 238 - 518

NOTE: Residue numbering includes N-terminal Met.

Mutation

Effect

Reference

E238A No GroES binding; Decreased OTC folding (release & aggregation) (1)

L259S No GroES binding; No OTC binding; No growth of LG6 strain (1)

V263S No GroES binding; No OTC binding; No growth of LG6 strain (1)

V264S Decreased ATPase; No GroES binding; No OTC binding
No growth of LG6 strain (1)

N265A No GroES binding; Decreased OTC folding (no release)
No growth of LG6 strain (1)

K277E Decreased ATPase; Small colonies of LG6 strain (1)

F281D Decreased ATPase; decreased OTC folding (release & aggregation)
No growth of LG6 strain (1)

D283K No effect detected (1)

K286E No effect detected (1)

A293E No effect detected (1)

L309K Decreased OTC refolding (decreased release); No growth of LG6 strain (1)

L314K Decreased OTC refolding (decreased release) (1)

G337S/I349E Decreased OTC refolding (released & aggregation)
No growth of LG6 strain (1)

E338K No effect detected (1)

Y360E Decreased ATPase; No growth of LG6 strain (1)

D361K No GroES binding; Decreased OTC refolding (decreased release) (1)

R368A No effect detected (1)

K371E No effect detected (1)

V381S No effect detected (1)

A383E No ATPase; No GroES binding; No OTC refolding (no release)
No growth of LG6 strain (1)

K390A/K393A No effect detected (1)

H401F No effect detected (1)

A405E No ATPase; No OTC refolding (no release); No growth of LG6 strain (1)

A406E No ATPase; No GroES binding; Decreased OTC binding
Decreased OTC refolding, No growth of LG6 strain (1)

E409A No effect detected (1)

E461K Decreased OTC refolding (decreased release); No growth of LG6 strain (1)

C518S Decreased ATPase; Decreased GroEL stability (2)

C518S Decreased GroEL stability in presence of ATP or ADP (3)

C518S/C137S This double mutant reverses the effects of the C518S
mutant listed just above (3)

Mutation	Effect	Reference
E238A	No GroES binding; Decreased OTC folding (release & aggregation)	(1)
L259S	No GroES binding; No OTC binding; No growth of LG6 strain	(1)
V263S	No GroES binding; No OTC binding; No growth of LG6 strain	(1)
V264S	Decreased ATPase; No GroES binding; No OTC binding No growth of LG6 strain	(1)
N265A	No GroES binding; Decreased OTC folding (no release) No growth of LG6 strain	(1)
K277E	Decreased ATPase; Small colonies of LG6 strain	(1)
F281D	Decreased ATPase; decreased OTC folding (release & aggregation) No growth of LG6 strain	(1)
D283K	No effect detected	(1)
K286E	No effect detected	(1)
A293E	No effect detected	(1)
L309K	Decreased OTC refolding (decreased release); No growth of LG6 strain	(1)
L314K	Decreased OTC refolding (decreased release)	(1)
G337S/I349E	Decreased OTC refolding (released & aggregation) No growth of LG6 strain	(1)
E338K	No effect detected	(1)
Y360E	Decreased ATPase; No growth of LG6 strain	(1)
D361K	No GroES binding; Decreased OTC refolding (decreased release)	(1)
R368A	No effect detected	(1)
K371E	No effect detected	(1)
V381S	No effect detected	(1)
A383E	No ATPase; No GroES binding; No OTC refolding (no release) No growth of LG6 strain	(1)
K390A/K393A	No effect detected	(1)
H401F	No effect detected	(1)
A405E	No ATPase; No OTC refolding (no release); No growth of LG6 strain	(1)
A406E	No ATPase; No GroES binding; Decreased OTC binding Decreased OTC refolding, No growth of LG6 strain	(1)
E409A	No effect detected	(1)
E461K	Decreased OTC refolding (decreased release); No growth of LG6 strain	(1)
C518S	Decreased ATPase; Decreased GroEL stability	(2)
C518S	Decreased GroEL stability in presence of ATP or ADP	(3)
C518S/C137S	This double mutant reverses the effects of the C518S mutant listed just above	(3)

This information is accurate to the best of my knowledge. However, you should check the references cited for a more complete understanding of these mutations and their effects.

References

Fenton et al (1994) Nature 371: 614-619.
Luo & Horowitz (1994) J. Biol. Chem. 269: 32151-32154.
Horovitz et al (1994) J. Mol. Biol. 238: 133-138.

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Last Updated April 11, 1996