GroEL/GroES Mutational Database

Mutations in GroEL - Page 1

Residues 1 - 237



NOTE: Residue numbering includes N-terminal Met.
Mutations are listed in sequence order (1-548).



Mutation

Effect

Reference
A3S Increased cooperativity of ATP hydrolysis
Decreased GroEL stability
(5)
K4E Dissociation of oligomer
(6)
K4I Decreased GroEL stability
(6)
K4R Decreased GroEL stability
(6)
R13G Decrease in ATPase activity
(1)
R13G/A126V Decrease in ATPase activity
(1)
D25R Decrease in OTC refolding; Small colonies of LG6 strain
(1)
F44A Decrease in ATPase activity; No growth of LG6 strain
(1)
G45E Decrease in OTC refolding; Small colonies of LG6 strain
(1)
S55E No effect detected
(1)
K65E No effect detected
(1)
E76K No effect detected
(1)
D87K Decreased GroES binding; Decreased OTC folding (no release)
No growth of LG6 strain
(1)
D87E Decreased ATP hydrolysis; Decreased polypeptide binding
Decreased Rubisco refolding
(4)
D87N Decreased GroES binding; Decreased OTC folding (no release)
No growth of LG6 strain
(1)
A96E No effect detected
(1)
V107E No effect detected
(1)
I150E No ATPase, No GroES binding, No OTC refolding (no release)
No growth of LG6 strain
(1)
S151V No ATPase, No GroES binding, No OTC refolding (no release)
No growth of LG6 strain
(1)
A152E No ATPase, No GroES binding, No OTC refolding (no release)
Tiny colonies in LG6 strain
(1)
D196A No effect detected
(1)
R197A No effect detected
(1)
R197A Decreased positive cooperativity in ATP hydrolysis
Strong substrate (ATP) inhibition
(2)
Y199E No GroES binding, No OTC binding, No growth of LG6 strain
(1)
L200E No effect detected
(1)
S201E Decreased OTC binding, Decreased OTC refolding
No growth of LG6 strain
(1)
S201F Decreased ATPase activity, Decreased GroES binding
Decrease in GroEL stability
(3)
Y203E No GroES binding, No OTC binding, No growth of LG6 strain
(1)
F204E No GroES binding, No OTC binding, Tiny colonies in LG6 strain
(1)
K225E Small colonies in LG6 strain
(1)
I227S No effect detected
(1)
I230S No effect detected
(1)
L234E Decreased ATPase, No GroES binding
No OTC binding, No growth of LG6 strain
(1)
L237E No GroES binding, No OTC binding, No growth of LG6 strain
(1)



This information is accurate to the best of my knowledge. However, you should check the references cited for a more complete understanding of these mutations and their effects.




References


  1. Fenton et al (1994) Nature 371:614-619.
  2. Yifrach et al (1994) J. Mol. Biol. 243:397-401.
  3. Baneyx & Gatenby (1992) J. Biol. Chem. 267:11637-11644.
  4. Weiss & Goloubinoff (1995) J. Biol. Chem. 270:13956-13960.
  5. Horovitz et al (1993) J. Mol. Biol. 231:58-64.
  6. Horovitz et al (1993) J. Biol. Chem. 268:9957-9959.




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